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5, 1, 2, 4, 8, 16 and 24 h post-administration. The tissues were immediately washed twice with normal saline, wiped with filter paper, weighed and homogenized with 1. 0 ml of normal saline. The tissue samples were centrifuged at 2583 x g for 15 minutes. Quantitative analysis by HPLC For MBZ assay, 0.